LOCUS       40924_2632        5167 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector p35S-loxP-Zeo DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Aoyama T, Hiwatashi Y, Hasebe M.
  TITLE     Moss transformation vector p35S-loxP-Zeo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Aoyama T, Hiwatashi Y, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2010) Contact:Yuji Hiwatashi National Institute 
            for Basic Biology, Evolutionary Biology; Myodaiji, Nishigonaka, 38, 
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 5167)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5167)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JAN-2010) Contact:Yuji Hiwatashi National Institute for Basic 
            Biology, Evolutionary Biology; Myodaiji, Nishigonaka, 38, Okazaki, 
            Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    708..741
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     708..714
                     /label=loxP
                     /note="loxP"
     primer_bind     816..832
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(1344..1520)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1593..1964)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVLVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1971..2313)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    complement(2854..2887)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2979..2997)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3018..3034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3042..3058)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3066..3096)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3111..3132)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3420..4008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4182..5039)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5040..5144)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattccata acttcgtata
      721 gcatacatta tacgaagtta tgccccccta ctccaaaaat gtcaaagata cagtctcaga
      781 agaccaaagg gctattgaca cttttcaaca aagggtctag aactagtgga tccccgtcac
      841 cggtgtgagg gaactagttt tgatcttgaa agatctttta tctttagagt taagaactct
      901 ttcgtatttt ggtgaggttt tatcctcttg agttttggtc atagacctat tcatggctct
      961 gataccaatt tttaagcggg ggcttatgcg gattatttct taaattgata aggggttatt
     1021 agggggtata gggtataaat acaagcattc ccttagcgta tagtataagt atagtagcgt
     1081 acctctatca aatttccatc ttcttacctt gcacagggcc tgcaacctta tccttccttg
     1141 tcttcctcct tccttccgtc cacttcatca tatttaaacc aaacctacgg gggagtcaac
     1201 gtaaccaacc ctgccttagc atcttttccc taacggcctc ctgcctaagc ggtacttcta
     1261 gcttcgaacg gcgtctgggc tccaggttta gtcgtctcgt gtctggttta tattcacgac
     1321 aaagatctat agggacttta ggagatctgg attttagtac tggattttgg ttttaggaat
     1381 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     1441 tgctcaacac atgagcgaaa ccctataaga accctaattt cccttatcgg gaaactactc
     1501 acacattatt tatggagaaa atagagagag atagatttgt agagagagac tggtgatttc
     1561 agcgtaccgt ccgaacccca gagtcccgct cagtcctgct cctcggccac gaagtgcacg
     1621 cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg
     1681 gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg
     1741 tacagctcgt ccaggccgcg caccaacacc caggccaggg tgttgtccgg caccacctgg
     1801 tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc
     1861 tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg
     1921 tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccatggatcc cctctccaaa
     1981 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
     2041 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
     2101 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     2161 gaggcatctt caacgatggc ctttccttta tcgcaatgat ggcatttgta ggagccacct
     2221 tccttttcca ctatcttcac aataaagtga cagatagctg ggcaatggaa tccgaggagg
     2281 tttccggata ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc tgagactgta
     2341 tctttgatat ttttggagta gacaagtgtg tcgtgctcca ccatgttgac gaagattttc
     2401 ttcttgtcat tgagtcgtaa gagactctgt atgaactgtt cgccagtctt tacggcgagt
     2461 tctgttaggt cctctatttg aatctttgac tccatggcct ttgattcagt gggaactacc
     2521 tttttagaga ctccaatctc tattacttgc cttggtttgt gaagcaagcc ttgaatcgtc
     2581 catactggaa tagtacttct gatcttgaga aatatatctt tctctgtgtt cttgatgcag
     2641 ttagtcctga atcttttgac tgcatcttta accttcttgg gaaggtattt gatttcctgg
     2701 agattattgc tcgggtagat cgtcttgatg agacctgctg cgtaagcctc tctaaccatc
     2761 tgtgggttag cattctttct gaaagggaat tcctcaccaa aatacgaaag agttcttaac
     2821 tctaaagata aaagatcttt caagatcaaa actataactt cgtatagcat acattatacg
     2881 aagttatccc tcacaccggt gacggggatc gcatgcccgg gtctagacat atggatccta
     2941 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     3001 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3061 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3121 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3181 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3241 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3601 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3841 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4081 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4141 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4201 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4261 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4321 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4381 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4441 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4501 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4561 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4621 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4681 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4741 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4801 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4861 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4921 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4981 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5041 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5101 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5161 gtgccac
//
